Analytical Data
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Gene name
mrkD
- Application
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Species
Klebsiella pneumoniae
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P21648
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Expression Region
19-321aa
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Molecular Weight
39.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the MrkD recombinant protein has garnered significant interest due to its potential role in understanding bacterial adhesion and biofilm formation, particularly in uropathogenic strains of Escherichia coli. MrkD is a critical adhesin protein associated with type 3 fimbriae, which facilitate the attachment of bacteria to host tissues and surfaces, thereby contributing to the pathogenicity of urinary tract infections. Research into MrkD not only helps unveil the mechanisms underlying bacterial colonization but also offers insights into the development of therapeutic strategies, such as vaccines or inhibitors that could disrupt the adhesion process. Furthermore, the recombinant expression of MrkD allows for the generation of sufficient quantities of the protein for advanced studies, enabling researchers to analyze its structure, function, and interaction with host cells in detail. As antibiotic resistance continues to rise, understanding proteins like MrkD is essential for developing novel approaches to combat bacterial infections and enhance our overall grasp of bacterial pathogenesis.
