Analytical Data
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Gene name
CHMP4A
- Application
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Alternative Names
CHMP4A；C14orf123；SHAX2；Charged multivesicular body Protein 4a
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BY43
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Expression Region
1-222aa
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AA Sequence
MSGLGRLFGK GKKEKGPTPE EAIQKLKETE KILIKKQEFL EQKIQQELQT AKKYGTKNKR AALQALRRKK RFEQQLAQTD GTLSTLEFQR EAIENATTNA EVLRTMELAA QSMKKAYQDM DIDKVDELMT DITEQQEVAQ QISDAISRPM GFGDDVDEDE LLEELEELEQ EELAQELLNV GDKEEEPSVK LPSVPSTHLP AGPAPKVDED EEALKQLAEW VS
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CHMP4A, or Chromatin-modifying protein 4A, is a critical component of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery, which plays a vital role in various cellular processes, including multivesicular body formation, cell division, and the repair of damaged membranes. Understanding CHMP4A's function is pivotal in cell biology and has implications for diseases such as cancer and neurodegenerative disorders, where dysfunctional protein sorting and membrane trafficking are implicated. Recent studies have highlighted the importance of CHMP4A in regulating intracellular signaling pathways, particularly those involved in the degradation of membrane proteins, which can affect cellular homeostasis and response to external stimuli. Given its involvement in essential cellular functions, recombinant CHMP4A protein has emerged as a valuable tool for elucidating its mechanisms of action and interactions within the ESCRT complex. Researchers are increasingly focusing on characterizing the structure, dynamics, and regulatory mechanisms of CHMP4A to develop therapeutic strategies that target its pathways. The production and study of recombinant CHMP4A protein facilitate a deeper understanding of its biological roles and its potential as a target for pharmacological intervention. Overall, the study of CHMP4A and its recombinant forms represents a significant frontier in the quest to decode the intricate processes governing cellular organization and response to stress, holding promise for innovative therapeutic avenues in various prevalent diseases.
