Analytical Data
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Gene name
GDF9
- Application
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Alternative Names
GDF9；Growth/differentiation factor 9
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60383
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Expression Region
1-454aa
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AA Sequence
MARPNKFLLWFCCFAWLCFPISLGSQASGGEAQIAASAELESGAMPWSLL QHIDERDRAGLLPALFKVLSVGRGGSPRLQPDSRALHYMKKLYKTYATKE GIPKSNRSHLYNTVRLFTPCTRHKQAPGDQVTGILPSVELLFNLDRITTV EHLLKSVLLYNINNSVSFSSAVKCVCNLMIKEPKSSSRTLGRAPYSFTFN SQFEFGKKHKWIQIDVTSLLQPLVASNKRSIHMSINFTCMKDQLEHPSAQ NGLFNMTLVSPSLILYLNDTSAQAYHSWYSLHYKRRPSQGPDQERSLSAY PVGEEAAEDGRSSHHRHRRGQETVSSELKKPLGPASFNLSEYFRQFLLPQ NECELHDFRLSFSQLKWDNWIVAPHRYNPRYCKGDCPRAVGHRYGSPVHT MVQNIIYEKLDSSVPRPSCVPAKYSPLSVLTIEPDGSIAYKEYEDMIATK CTCR
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GDF9 (Growth Differentiation Factor 9) is a member of the TGF-beta superfamily and plays a crucial role in ovarian development and folliculogenesis. Initially identified as a factor involved in the regulation of oocyte growth and maturation, GDF9 has garnered significant attention in reproductive biology and developmental research. Studies have shown that GDF9 is essential for the formation of ovarian follicles and influences granulosa cell function, thereby impacting fertility. The recombinant production of GDF9 allows for detailed investigations into its biological activities and potential therapeutic applications. Utilizing techniques such as DNA cloning and recombinant expression systems, researchers have successfully produced GDF9 in various host cells, enabling in vitro functional assays and elucidation of its signaling pathways. These studies aim to uncover the mechanisms by which GDF9 facilitates ovarian function and its potential implications in treating female infertility and ovarian disorders. Moreover, the ability to produce recombinant GDF9 also opens avenues for exploring its role in other physiological processes and may contribute to advances in reproductive technologies and regenerative medicine.
