Analytical Data
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Gene name
USP18
- Application
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Alternative Names
(43 kDa ISG15-specific protease)(hUBP43)(ISG15-specific-processing protease)(Ubl thioesterase 18)
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Species
Human
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UMW8
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Expression Region
1-372aa
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Molecular Weight
50.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
USP18 (Ubiquitin-specific protease 18) is an essential deubiquitinating enzyme that plays a crucial role in various cellular processes, including the regulation of the immune response, cell differentiation, and development. It is known for its ability to reverse ubiquitination, a post-translational modification that typically targets proteins for degradation, thus influencing protein stability and function. USP18 is particularly important in the context of type I interferon signaling, as it modulates the antiviral response by deubiquitinating pathway components and preventing excessive inflammatory responses. Dysregulation of USP18 has been implicated in several diseases, including autoimmune disorders and cancer, highlighting its potential as a therapeutic target. The study of recombinant USP18 proteins aims to elucidate its enzymatic mechanisms, structural properties, and interactions with substrates. This research could provide insights into its role in disease pathology and aid in the development of novel therapeutic strategies aimed at modulating USP18 activity for clinical benefit. Understanding USP18 function at the molecular level also contributes to the broader field of ubiquitin biology and helps clarify the complex regulatory networks that govern cellular homeostasis. As such, the production and characterization of recombinant USP18 proteins are crucial for advancing our understanding of its multifaceted roles in health and disease.
