Analytical Data
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Gene name
CHMP1B
- Application
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Alternative Names
CHMP1B；C18orf2；Charged multivesicular body Protein 1b
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7LBR1
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Expression Region
1-199aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSNMEK HLFNLKFAAK ELSRSAKKCD KEEKAEKAKI KKAIQKGNME VARIHAENAI RQKNQAVNFL RMSARVDAVA ARVQTAVTMG KVTKSMAGVV KSMDATLKTM NLEKISALMD KFEHQFETLD VQTQQMEDTM SSTTTLTTPQ NQVDMLLQEM ADEAGLDLNM ELPQGQTGSV TSVASAEQD ELSQRLARLR DQV
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Molecular Weight
25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CHMP1B, a member of the CHMP (Charged MVB Protein) family, plays a crucial role in the endosomal sorting pathways, particularly in the formation of multivesicular bodies (MVBs) and the regulation of protein degradation via the ESCRT (Endosomal Sorting Complex Required for Transport) machinery. Research on CHMP1B has gained significant interest due to its involvement in various cellular processes, including membrane dynamics, protein sorting, and response to cellular stress. Impairments in CHMP1B function are associated with several diseases, including neurodegenerative disorders, where abnormal endosomal trafficking contributes to pathogenesis. Given its essential roles, recombinant CHMP1B protein is valuable for studying its biochemical properties, interaction partners, and functional implications in cellular mechanisms. Recent advancements in recombinant protein technology have enabled the production of CHMP1B at a high purity, facilitating in vitro assays and structural analyses. Additionally, understanding the dynamics of CHMP1B can shed light on the molecular basis of diseases linked to endosomal dysfunctions, making it a promising target for therapeutic intervention. The research on CHMP1B not only enriches the fundamental knowledge of intracellular processes but also opens avenues for developing novel strategies to address diseases associated with ESCRT and endosomal sorting abnormalities.
