Analytical Data
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基因名
GHITM
- Application
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别名
GHITM；DERP2；MICS1；TMBIM5；Growth hormone-inducible transmembrane Protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H3K2
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表达区间
1-345aa
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氨基酸序列
MLAARLVCLRTLPSRVFHPAFTKASPVVKNSITKNQWLLTPSREYATKTRIGIRRGRTGQELKEAALEPSMEKIFKIDQMGRWFVAGGAAVGLGALCYYGLGLSNEIGAIEKAVIWPQYVKDRIHSTYMYLAGSIGLTALSAIAISRTPVLMNFMMRGSWVTIGVTFAAMVGAGMLVRSIPYDQSPGPKHLAWLLHSGVMGAVVAPLTILGGPLLIRAAWYTAGIVGGLSTVAMCAPSEKFLNMGAPLGVGLGLVFVSSLGSMFLPPTTVAGATLYSVAMYGGLVLFSMFLLYDTQKVIKRAEVSPMYGVQKYDPINSMLSIYMDTLNIFMRVATMLATGGNRKK
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分子量
37.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The research on GHITM (Glutamate Transporter Homologue from Escherichia coli) recombinant protein stems from the need to elucidate the mechanisms of glutamate transport and its implications in various physiological and pathological processes. Glutamate is a crucial neurotransmitter in the central nervous system, playing a vital role in synaptic transmission, learning, and memory. Dysregulation of glutamate transporters is linked to several neurological disorders, including Alzheimer's disease, schizophrenia, and epilepsy. GHITM, which is homologous to known mammalian glutamate transporters, provides a unique opportunity to study the structural and functional characteristics of this transporter in a simpler prokaryotic system. By expressing and purifying GHITM as a recombinant protein, researchers aim to analyze its transport properties, substrate specificity, and regulatory mechanisms. This study not only enhances our understanding of glutamate handling in cells but also holds potential for developing therapeutic strategies targeting glutamate dysregulation in neurological diseases. The successful characterization of GHITM may serve as a foundation for future research on other glutamate transporters and their roles in health and disease.
