Analytical Data
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Gene name
IFN-alpha 2/IFNA2
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简介
IFN-alpha 2 (IFNA2), belongs to type I interferon family, is a protein secreted by cells infected by a virus and acting on other cells to inhibit viral infection[1]. IFN-alpha 2 increases the frequencies of cytotoxic CD8+ T cells and induces CD4+ T cell depletion[3]. IFN-alpha 2a/IFNA2 Protein, Human (C24-E188, R46K) contains 165 a.a., is produced in E. coli with tag free.
- Application
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Biological Activity
The ED50 is ≤0.5005 ng/mL as measured by TF-1 cells, corresponding to a specific activity of ≥1.998 × 106 units/mg. Measured by a cytotoxicity assay using TF-1 Cells. The ED50 for this effect is 0.1509 ng/mL, corresponding to a specific activity is 6.627 ×106units/mg.
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Alternative Names
rHuIFN-α2a; IFNA; IFNA2; IFN-a 2a
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P01563
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Expression Region
C24-E188, R46K
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Protein Length
Full Length of Mature Protein
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Molecular Weight
16 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interferon-alpha 2 (IFN-alpha 2, or IFNA2) is a type of cytokine that plays a crucial role in the immune response against viral infections and malignancies. It is particularly known for its antiviral, antiproliferative, and immunomodulatory effects, making it an essential component in therapeutic strategies for various diseases, including hepatitis C and certain types of cancer. The recombinant form of IFN-alpha 2 has been extensively studied and developed for clinical use, providing targeted therapeutic benefits while minimizing systemic side effects. Research has shown that IFNA2 can enhance the activity of immune cells, such as natural killer (NK) cells and T lymphocytes, which are vital for the body’s defense mechanisms. Moreover, its potential synergistic effects when combined with other medications have opened avenues for innovative treatment regimens. Given the rising prevalence of viral infections and cancers worldwide, continued research into IFN-alpha 2, including the optimization of its recombinant production and understanding its molecular mechanisms, remains a high priority in biomedical research. As understanding of IFNA2's role in modulating immune responses deepens, it may lead to novel therapeutic applications and improved patient outcomes in the fight against infectious diseases and cancer.
