Analytical Data
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Gene name
PIGX
- Application
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Alternative Names
GPI mannosyltransferase subunit; Phosphatidylinositol glycan class X; Phosphatidylinositol-glycan biosynthesis class X protein; PIG-X; Pigx; PIGX_HUMAN
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Species
Human
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TBF5
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Expression Region
42-230aa
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Molecular Weight
37.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIGX (Phosphatidylinositol Glycan Anchor Biosynthesis Class X) is a crucial protein involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors, which are essential for the tethering of proteins to the cell membrane. The study of PIGX is significant in understanding various diseases, particularly those related to cell signaling and membrane-related disorders. Mutations in the PIGX gene can disrupt GPI anchor synthesis, leading to severe clinical implications such as developmental disorders and immunological defects. Additionally, research on PIGX plays a vital role in the field of glycobiology, as GPI anchors are implicated in key physiological functions, including cell adhesion, signaling, and immune response. Investigating the molecular mechanisms of PIGX and its interactions with other components in the GPI anchor biosynthetic pathway can provide insights into potential therapeutic targets for treating related diseases. Consequently, the recombinant expression and purification of PIGX is of great interest, not only for basic scientific research but also for developing biopharmaceutical applications that harness the therapeutic potential of GPI-anchored proteins.
