Analytical Data
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基因名
CPT1A
- Application
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别名
CPT1A；CPT1；Carnitine O-palmitoyltransferase 1. liver isoform
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种属
Human
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表达系统
Cell-Free Expression
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标签
N-terminal His-Tag
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P50416
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表达区间
Full Length
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氨基酸序列
MAEAHQAVAF QFTVTPDGID LRLSHEALRQ IYLSGLHSWK KKFIRFKNGI ITGVYPASPS SWLIVVVGVM TTMYAKIDPS LGIIAKINRT LETANCMSSQ TKNVVSGVLF GTGLWVALIV TMRYSLKVLL SYHGWMFTEH GKMSRATKIW MGMVKIFSGR KPMLYSFQTS LPRLPVPAVK DTVNRYLQSV RPLMKEEDFK RMTALAQDFA VGLGPRLQWY LKLKSWWATN YVSDWWEEYI YLRGRGPLMV NSNYYAMDLL YILPTHIQAA RAGNAIHAIL LYRRKLDREE IKPIRLLGST IPLCSAQWER MFNTSRIPGE ETDTIQHMRD SKHIVVYHRG RYFKVWLYHD GRLLKPREME QQMQRILDNT SEPQPGEARL AALTAGDRVP WARCRQAYFG RGKNKQSLDA VEKAAFFVTL DETEEGYRSE DPDTSMDSYA KSLLHGRCYD RWFDKSFTFV VFKNGKMGLN AEHSWADAPI VAHLWEYVMS IDSLQLGYAE DGHCKGDINP NIPYPTRLQW DIPGECQEVI ETSLNTANLL ANDVDFHSFP FVAFGKGIIK KCRTSPDAFV QLALQLAHYK DMGKFCLTYE ASMTRLFREG RTETVRSCTT ESCDFVRAMV DPAQTVEQRL KLFKLASEKH QHMYRLAMTG SGIDRHLFCL YVVSKYLAVE SPFLKEVLSE PWRLSTSQTP QQQVELFDLE NNPEYVSSGG GFGPVADDGY GVSYILVGEN LINFHISSKF SCPETDSHRF GRHLKEAMTD IITLFGLSSN SKK
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分子量
87kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
CPT1A, or Carnitine Palmitoyltransferase 1A, is a crucial enzyme involved in the mitochondrial oxidation of long-chain fatty acids. It catalyzes the transfer of an acyl group from CoA to carnitine, facilitating the transport of fatty acids into the mitochondria for β-oxidation. Research on CPT1A has gained significance due to its role in lipid metabolism and energy production, which are pivotal for maintaining cellular homeostasis and overall metabolic health. Dysregulation of CPT1A is implicated in various metabolic disorders, including obesity, diabetes, and cardiovascular diseases. Furthermore, CPT1A's involvement in the regulation of the liver's fat metabolism has positioned it as a potential therapeutic target for treating non-alcoholic fatty liver disease (NAFLD) and other related conditions. Recombinant CPT1A proteins are useful tools for studying the structure-function relationship of this enzyme, elucidating the mechanisms of its regulation, and testing potential inhibitors that could serve as pharmacological agents. As such, the exploration of CPT1A, particularly through recombinant protein studies, is essential to understanding its biological significance and therapeutic potential in metabolic diseases.
