Analytical Data
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Gene name
DHX9
- Application
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Alternative Names
DHX9；DDX9；LKP；NDH2；ATP-dependent RNA helicase A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q08211
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Expression Region
325-840aa
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AA Sequence
NQVGVVPWSPPQSNWNPWTSSNIDEGPLAFATPEQISMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISAVSVAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIVLMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFLEDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPETRLSMSQLNEKETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLEMNPHFGSHRYQILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLLRLGGIGQFLAKAIEPPPLDAVIEAEHTLRELD
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Molecular Weight
74.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DHX9, a member of the DEAH-box helicase family, plays a crucial role in various cellular processes, including RNA metabolism, transcription, and stress response. Its involvement in several biological functions has made it an important focus of research, particularly in the context of cancer and viral infections. Overexpression of DHX9 has been linked to tumor progression and poor prognosis in various cancers, suggesting its potential as a therapeutic target. Furthermore, DHX9 interacts with numerous RNA-binding proteins and contributes to the regulation of mRNA splicing and translation, highlighting its significance in post-transcriptional regulation. With the growing understanding of DHX9's role in disease mechanisms, generating recombinant DHX9 protein has become essential for exploring its biochemical properties, interactions, and regulatory mechanisms. This research aims to elucidate the functional aspects of DHX9, paving the way for developing novel therapeutic strategies that target its activity in pathological conditions. By employing techniques such as protein purification and functional assays, scientists aim to reveal the intricate details of DHX9's role in RNA biology, ultimately contributing to the broader understanding of gene expression regulation and its implications in health and disease.
