Analytical Data
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Gene name
rpsD
- Application
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Alternative Names
rpsD；ECF RNA polymerase sigma factor SigD
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A7V8
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Expression Region
2-206aa
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AA Sequence
ARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALELAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK
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Molecular Weight
27.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RpsD, a ribosomal protein, plays a crucial role in the assembly and function of the ribosomal subunit, an essential component of protein synthesis in all living organisms. It is a part of the 30S subunit in prokaryotic ribosomes and is involved in stabilizing the structure of rRNA and facilitating the binding of tRNA and mRNA during translation. Due to its importance in the ribosome's functionality, RpsD has garnered considerable attention in molecular biology and antibiotic research. Understanding the structure and function of RpsD can provide insights into the mechanisms of bacterial protein synthesis, revealing potential targets for novel antibiotics, especially amid the rising concerns of antibiotic resistance. Recombinant RpsD protein has been produced for detailed biochemical and structural studies, enabling researchers to explore its interactions with other ribosomal components and inhibitors. This research paves the way for potential therapeutic applications by facilitating the design of new drugs that can selectively target bacterial ribosomes without affecting eukaryotic cells. Overall, the study of RpsD and its recombinant forms is a promising avenue in the search for innovative solutions to combat bacterial infections and enhance our understanding of ribosome biology.
