Analytical Data
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Gene name
PAX2
- Application
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Alternative Names
PAX2；Paired box Protein Pax-2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q02962
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Expression Region
1-388aa
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AA Sequence
MDMHCKADPFSAMHPGHGGVNQLGGVFVNGRPLPDVVRQRIVELAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIKPGVIGGSKPKVATPKVVDKIAEYKRQNPTMFAWEIRDRLLAEGICDNDTVPSVSSINRIIRTKVQQPFHPTPDGAGTGVTAPGHTIVPSTASPPVSSASNDPVGSYSINGILGIPRSNGEKRKRDEDVSEGSVPNGDSQSGVDSLRKHLRADTFTQQQLEALDRVFERPSYPDVFQASEHIKSEQGNEYSLPALTPGLDEVKSSLSASTNPELGSNVSGTQTYPVVTGRDMASTTLPGYPPHVPPTGQGSYPTSTLAGMVPEAAVGPSSSLMSKPGRKLAEVPPCVQPTGASSPATRTATPSTRPTTRL
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Molecular Weight
68.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PAX2 is a member of the paired box family of transcription factors, playing a crucial role in the development of various tissues, particularly in the kidneys, eyes, and central nervous system. Abnormal expression or mutation of PAX2 has been linked to congenital disorders such as renal agenesis and ocular anomalies. Due to its significant roles in embryogenesis and organogenesis, PAX2 has gained attention as a potential therapeutic target for regenerative medicine and cancer. Researchers have focused on the recombinant expression of PAX2 to understand its functional properties, interaction with other proteins, and regulatory mechanisms. The production of PAX2 recombinant protein allows for detailed biochemical studies, including binding affinity assays and reporter gene analyses, which help elucidate its role in gene expression regulation. Furthermore, examining PAX2's structure through techniques like X-ray crystallography and NMR spectroscopy can provide insights into its DNA-binding domains and potential for specificity towards target genes. This research not only enhances our understanding of PAX2’s developmental functions but also paves the way for its application in clinical settings, particularly in correcting dysregulation associated with various diseases. Overall, the study of PAX2 recombinant protein is pivotal for unveiling the complexities of its biological pathways and exploring innovative therapeutic avenues.
