Analytical Data
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Gene name
NIS
- Application
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Alternative Names
NIS；NIS；Sodium/iodide cotransporter
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q92911
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Expression Region
1-643aa
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AA Sequence
MEAVETGERPTFGAWDYGVFALMLLVSTGIGLWVGLARGGQRSAEDFFTGGRRLAALPVGLSLSASFMSAVQVLGVPSEAYRYGLKFLWMCLGQLLNSVLTALLFMPVFYRLGLTSTYEYLEMRFSRAVRLCGTLQYIVATMLYTGIVIYAPALILNQVTGLDIWASLLSTGIICTFYTAVGGMKAVVWTDVFQVVVMLSGFWVVLARGVMLVGGPRQVLTLAQNHSRINLMDFNPDPRSRYTFWTFVVGGTLVWLSMYGVNQAQVQRYVACRTEKQAKLALLINQVGLFLIVSSAACCGIVMFVFYTDCDPLLLGRISAPDQYMPLLVLDIFEDLPGVPGLFLACAYSGTLSTASTSINAMAAVTVEDLIKPRLRSLAPRKLVIISKGLSLIYGSACLTVAALSSLLGGGVLQGSFTVMGVISGPLLGAFILGMFLPACNTPGVLAGLGAGLALSLWVALGATLYPPSEQTMRVLPSSAARCVALSVNASGLLDPALLPANDSSRAPSSGMDASRPALADSFYAISYLYYGALGTLTTVLCGALISCLTGPTKRSTLAPGLLWWDLARQTASVAPKEEVAILDDNLVKGPEELPTGNKKPPGFLPTNEDRLFFLGQKELEGAGSWTPCVGHDGGRDQQETNL
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Molecular Weight
68.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NIS (Sodium/Iodide Symporter) is a membrane protein crucial for the uptake of iodide in thyroid cells, playing a pivotal role in thyroid hormone synthesis. Its expression and functional integrity are essential for the proper functioning of the thyroid gland, and dysregulation can lead to various thyroid disorders, including goiter and cancer. Research into the recombinant expression of NIS has gained momentum due to its potential applications in both basic research and clinical settings. By producing NIS as a recombinant protein, scientists aim to elucidate its structural and functional properties, which can lead to a better understanding of its mechanism of action, the identification of regulatory elements, and the development of novel therapeutic strategies. Moreover, the availability of purified NIS protein enables detailed studies on its interaction with other proteins within the thyroid, assisting in the exploration of its role in iodide metabolism and transport. Additionally, recombinant NIS has implications beyond thyroid research, as it is being studied for its potential use in targeted radionuclide therapy for thyroid cancer and other malignancies, where it can facilitate the selective uptake of therapeutic radioisotopes. This multifaceted research underscores the importance of NIS in both physiology and medicine, highlighting the need for continued investigation into its recombinant expression and functional characterization.
