Analytical Data
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基因名
EIF3F
- Application
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别名
EIF3F；EIF3S5;Eukaryotic translation initiation factor 3 subunit F
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种属
Human
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表达系统
E. coli
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标签
N-terminal His Tag
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00303
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表达区间
2-357aa
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氨基酸序列
GSSHHHHHH SSGLVPRGSH MGSMATPAVP VSAPPATPTP VPAAAPASVP APTPAPAAAP VPAAAPASSS DPAAAAAATA APGQTPASAQ APAQTPAPAL PGPALPGPFP GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL
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分子量
41.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
EIF3F, a crucial component of the eukaryotic translation initiation factor 3 (eIF3), plays a significant role in the initiation of protein synthesis by facilitating the assembly of the translation initiation complex. Its primary function is to stabilize the interaction between the mRNA and the ribosome, which is essential for the accurate translation of genetic information into functional proteins. Research on EIF3F has gained popularity due to its implications in various cellular processes and its potential links to diseases, including cancer. Abnormal expression of EIF3F has been associated with tumor progression and resistance to chemotherapy, making it a target for therapeutic interventions. The ability to produce recombinant EIF3F protein allows for detailed studies of its structure-function relationships, interactions with other translation factors, and role in the regulation of gene expression. Additionally, the development of EIF3F-related assays can facilitate drug discovery efforts aimed at modulating its activity in disease contexts. As such, the investigation of EIF3F and its recombinant protein forms is crucial for understanding the molecular underpinnings of translation regulation and its broader implications in health and disease.
