Analytical Data
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基因名
RARS
- Application
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别名
RARS；RARS；Arginine--tRNA ligase. cytoplasmic
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P54136
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表达区间
1-660aa
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氨基酸序列
MDVLVSECSARLLQQEEEIKSLTAEIDRLKNCGCLGASPNLEQLQEENLK LKYRLNILRKSLQAERNKPTKNMINIISRLQEVFGHAIKAAYPDLENPPL LVTPSQQAKFGDYQCNSAMGISQMLKTKEQKVNPREIAENITKHLPDNEC IEKVEIAGPGFINVHLRKDFVSEQLTSLLVNGVQLPALGENKKVIVDFSS PNIAKEMHVGHLRSTIIGESISRLFEFAGYDVLRLNHVGDWGTQFGMLIA HLQDKFPDYLTVSPPIGDLQVFYKESKKRFDTEEEFKKRAYQCVVLLQGK NPDITKAWKLICDVSRQELNKIYDALDVSLIERGESFYQDRMNDIVKEFE DRGFVQVDDGRKIVFVPGCSIPLTIVKSDGGYTYDTSDLAAIKQRLFEEK ADMIIYVVDNGQSVHFQTIFAAAQMIGWYDPKVTRVFHAGFGVVLGEDKK KFKTRSGETVRLMDLLGEGLKRSMDKLKEKERDKVLTAEELNAAQTSVAY GCIKYADLSHNRLNDYIFSFDKMLDDRGNTAAYLLYAFTRIRSIARLANI DEEMLQKAARETKILLDHEKEWKLGRCILRFPEILQKILDDLFLHTLCDY IYELATAFTEFYDSCYCVEKDRQTGKILKVNMWRMLLCEAVAAVMAKGFD ILGIKPVQRM
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分子量
99 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RARS (Arginyl-tRNA synthetase) is a critical enzyme that plays a significant role in protein synthesis by catalyzing the attachment of arginine to its corresponding tRNA, essential for the fidelity of translation within the cell. Research into RARS has gained momentum due to its implications in various physiological and pathological processes, including neurodegenerative diseases, cancer, and immune responses. Specifically, mutations in the RARS gene have been linked to certain conditions such as Charcot-Marie-Tooth disease, highlighting the importance of understanding its function and regulation. Beyond its role in translation, RARS is also involved in non-canonical functions, such as modulating signaling pathways and influencing cellular stress responses. This multi-faceted role positions RARS as a compelling target for therapeutic interventions. Consequently, studies have focused on characterizing the enzyme's structure, regulatory mechanisms, and potential as a biomarker or drug target. Advances in recombinant protein technology have enabled the production and purification of RARS, facilitating further investigation into its enzymatic activity and interactions with other cellular components. Understanding RARS at a molecular level could provide insights into its contribution to disease mechanisms and open up avenues for innovative treatments that leverage its unique properties. Overall, the study of RARS not only enhances our comprehension of fundamental biological processes but also underscores its potential relevance in clinical settings.
