Analytical Data
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Gene name
FH
- Application
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Alternative Names
FH;Fumarate hydratase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P07954
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Expression Region
1-510aa
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AA Sequence
MYRALRLLARSRPLVRAPAAALASAPGLGGAAVPSFWPPNAARMASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The FH (fumarate hydratase) gene plays a crucial role in the Krebs cycle, participating in the conversion of fumarate to malate and thus influencing cellular metabolism. Mutations in the FH gene are linked to hereditary leiomyomatosis and renal cell carcinoma (HLRCC), a condition characterized by the development of smooth muscle tumors and a predisposition to specific types of kidney cancer. Research has increasingly focused on FH as a target for understanding cancer metabolism, particularly because fumarate accumulation due to FH deficiency can lead to cellular stress and activation of hypoxia-inducible factors (HIFs), promoting tumorigenesis. Recombinant FH proteins have been engineered for various applications, including studying enzyme kinetics, exploring structure-function relationships, and testing potential therapeutic interventions. By utilizing FH in its recombined form, scientists aim to elucidate its biochemical properties and discover how its dysregulation contributes to cancer development. Additionally, the study of FH and its associated pathways may pave the way for new treatment strategies, targeting metabolic vulnerabilities in tumors. Hence, understanding the structure and function of recombinant FH proteins is pivotal for advancing both fundamental biochemical research and potential clinical applications in oncology.
