Analytical Data
-
Gene name
GAL4
- Application
-
Alternative Names
GAL4;Regulatory Protein GAL4
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P56470
-
Expression Region
1-323aa
-
AA Sequence
MAYVPAPGYQ PTYNPTLPYY QPIPGGLNVG MSVYIQGVAS EHMKRFFVNF VVGQDPGSDV AFHFNPRFDG WDKVVFNTLQ GGKWGSEERK RSMPFKKGAA FELVFIVLAE HYKVVVNGNP FYEYGHRLPL QMVTHLQVDG DLQLQSINFI GGQPLRPQGP PMMPPYPGPG HCHQQLNSLP TMEGPPTFNP PVPYFGRLQG GLTARRTIII KGYVPPTGKS FAINFKVGSS GDIALHINPR MGNGTVVRNS LLNGSWGSEE KKITHNPFGP GQFFDLSIRC GLDRFKVYAN GQHLFDFAHR LSAFQRVDTL EIQGDVTLSY VQI
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The GAL4 protein, originally derived from the yeast Saccharomyces cerevisiae, is a transcription factor that plays a crucial role in the regulation of gene expression. It binds to specific DNA sequences known as UAS (Upstream Activating Sequences) to activate the transcription of adjacent genes. The study of GAL4 has provided invaluable insights into the mechanisms of eukaryotic gene regulation and its utility in molecular biology, particularly in the development of gene expression systems. Researchers have harnessed GAL4's ability to promote gene activation in a variety of organisms through the creation of the GAL4-UAS system, which allows for targeted expression of genes in specific tissues or at specific developmental stages. This system has been widely utilized in model organisms, such as Drosophila melanogaster, for functional studies of genes and pathways, as it enables researchers to manipulate gene expression with spatial and temporal precision. Additionally, GAL4's role in the context of synthetic biology and gene therapy continues to expand, as scientists explore its potential for developing new tools for controlled gene expression. Overall, the research on GAL4 and its recombination protein has significantly advanced our understanding of gene regulation and opened new avenues for innovative applications in biotechnology and medicine.
