Analytical Data
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基因名
cry1Ia
- Application
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别名
cry1Ia；CGCryV；cryII(a)；cryV；Pesticidal crystal Protein Cry1Ia
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q45752
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表达区间
1-719aa
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氨基酸序列
MKLKNQDKHQSFSSNAKVDKISTDSLKNETDIELQNINHEDCLKMSEYENVEPFVSASTIQTGIGIAGKILGTLGVPFAGQVASLYSFILGELWPKGKNQWEIFMEHVEEIINQKISTYARNKALTDLKGLGDALAVYHDSLESWVGNRNNTRARSVVKSQYIALELMFVQKLPSFAVSGEEVPLLPIYAQAANLHLLLLRDASIFGKEWGLSSSEISTFYNRQVERAGDYSDHCVKWYSTGLNNLRGTNAESWVRYNQFRRDMTLMVLDLVALFPSYDTQMYPIKTTAQLTREVYTDAIGTVHPHPSFTSTTWYNNNAPSFSAIEAAVVRNPHLLDFLEQVTIYSLLSRWSNTQYMNMWGGHKLEFRTIGGTLNISTQGSTNTSINPVTLPFTSRDVYRTESLAGLNLFLTQPVNGVPRVDFHWKFVTHPIASDNFYYPGYAGIGTQLQDSENELPPEATGQPNYESYSHRLSHIGLISASHVKALVYSWTHRSADRTNTIEPNSITQIPLVKAFNLSSGAAVVRGPGFTGGDILRRTNTGTFGDIRVNINPPFAQRYRVRIRYASTTDLQFHTSINGKAINQGNFSATMNRGEDLDYKTFRTVGFTTPFSFLDVQSTFTIGAWNFSSGNEVYIDRIEFVPVEVTYEAEYDFEKAQEKVTALFTSTNPRGLKTDVKDYHIDQVSNLVESLSDEFYLDEKRELFEIVKYAKQLHIERNM
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分子量
81.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cry1Ia is a protein derived from the bacterium Bacillus thuringiensis (Bt), which is known for its insecticidal properties. This protein belongs to the Cry toxin family, which is widely studied for its ability to control agricultural pests in a sustainable manner. The increasing reliance on chemical pesticides and the consequent environmental and health concerns have driven researchers to explore biopesticides like Cry proteins as safer alternatives. Cry1Ia specifically has garnered attention due to its effectiveness against a range of economically important lepidopteran pests. Understanding the structure and function of Cry1Ia at the molecular level can provide insights into its mechanism of action, enabling the development of genetically modified crops that express this protein for enhanced pest resistance. Additionally, research into Cry1Ia can facilitate the improvement of protein expression systems and the optimization of its insecticidal efficacy. The study of Cry1Ia not only contributes to agricultural biotechnology but also enriches our understanding of protein interactions in the context of insect physiology. As agricultural practices evolve towards more integrated pest management strategies, the ongoing exploration of Cry1Ia and its potential applications are vital for fostering sustainable agriculture and reducing the negative impacts associated with synthetic pesticides.
