Analytical Data
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基因名
GPC3
- Application
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别名
GPC3;OCI5;Glypican-3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P51654
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表达区间
25-559aa
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氨基酸序列
QPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVH
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分子量
62.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Glypican-3 (GPC3) is a heparan sulfate proteoglycan that plays a crucial role in various biological processes, including cell growth, development, and metabolism. It is predominantly expressed during embryonic development and is often downregulated in adult tissues, making its aberrant expression particularly significant in certain diseases. Notably, GPC3 has emerged as a key biomarker and potential therapeutic target in hepatocellular carcinoma (HCC) and other malignancies, given its overexpression in various tumors. Research into GPC3 recombinant proteins has gained momentum, focusing on their role in tumorigenesis and potential utility in targeted therapies. These recombinant proteins are being explored for their ability to induce immune responses, serving as vaccines or therapeutic agents against cancer. Moreover, GPC3’s involvement in regulating the Hedgehog signaling pathway and its interaction with growth factors positions it as a critical component in understanding tumor microenvironments. Recent studies have also highlighted GPC3's potential in developing targeted drug delivery systems, which could improve treatment efficacy while minimizing side effects. As the understanding of GPC3's mechanistic roles deepens, the development of GPC3-based therapies and diagnostics holds promise for advancing cancer treatment strategies. Overall, the ongoing research into GPC3 recombinant proteins is paving the way for innovative approaches in oncology, with the potential to enhance patient outcomes significantly.
