Analytical Data
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Gene name
GPC3
- Application
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Alternative Names
GPC3;OCI5;Glypican-3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51654
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Expression Region
25-559aa
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AA Sequence
QPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVH
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Molecular Weight
62.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Glypican-3 (GPC3) is a heparan sulfate proteoglycan that plays a crucial role in various biological processes, including cell growth, development, and metabolism. It is predominantly expressed during embryonic development and is often downregulated in adult tissues, making its aberrant expression particularly significant in certain diseases. Notably, GPC3 has emerged as a key biomarker and potential therapeutic target in hepatocellular carcinoma (HCC) and other malignancies, given its overexpression in various tumors. Research into GPC3 recombinant proteins has gained momentum, focusing on their role in tumorigenesis and potential utility in targeted therapies. These recombinant proteins are being explored for their ability to induce immune responses, serving as vaccines or therapeutic agents against cancer. Moreover, GPC3’s involvement in regulating the Hedgehog signaling pathway and its interaction with growth factors positions it as a critical component in understanding tumor microenvironments. Recent studies have also highlighted GPC3's potential in developing targeted drug delivery systems, which could improve treatment efficacy while minimizing side effects. As the understanding of GPC3's mechanistic roles deepens, the development of GPC3-based therapies and diagnostics holds promise for advancing cancer treatment strategies. Overall, the ongoing research into GPC3 recombinant proteins is paving the way for innovative approaches in oncology, with the potential to enhance patient outcomes significantly.
