Analytical Data
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基因名
ANG3
- Application
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别名
ANG3；ANG3；ANG4；Angiopoietin-4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95841
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表达区间
24-491aa
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氨基酸序列
GQFKIKK INQRRYPRAT DGKEEAKKCA YTFLVPEQRI TGPICVNTKG QDASTIKDMI TRMDLENLKD VLSRQKREID VLQLVVDVDG NIVNEVKLLR KESRNMNSRV TQLYMQLLHE IIRKRDNSLE LSQLENKILN VTTEMLKMAT RYRELEVKYA SLTDLVNNQS VMITLLEEQC LRIFSRQDTH VSPPLVQVVP QHIPNSQQYT PGLLGGNEIQ RDPGYPRDLM PPPDLATSPT KSPFKIPPVT FINEGPFKDC QQAKEAGHSV SGIYMIKPEN SNGPMQLWCE NSLDPGGWTV IQKRTDGSVN FFRNWENYKK GFGNIDGEYW LGLENIYMLS NQDNYKLLIE LEDWSDKKVY AEYSSFRLEP ESEFYRLRLG TYQGNAGDSM MWHNGKQFTT LDRDKDMYAG NCAHFHKGGW WYNACAHSNL NGVWYRGGHY RSKHQDGIFW AEYRGGSYSL RAVQMMIKPI D
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ANG3, or angiogenin 3, is a member of the angiogenin family of proteins, which are crucial in various biological processes, including angiogenesis, neuroprotection, and cellular survival. While the most well-studied member, angiogenin (ANG), has established roles in promoting blood vessel formation and has implications in cancer and other diseases, ANG3 has garnered interest due to its potential unique functions and regulatory mechanisms in vascular biology. Research into ANG3 recombinant protein has been driven by the need to understand its structure-function relationships, interactions with RNA, and effects on gene expression, especially in the context of pathological conditions such as ischemia and neurodegenerative diseases. Initial studies suggest that ANG3 may play a role in modulating immune responses and inflammation, further expanding the scope of its biological significance. Investigating ANG3 through recombinant protein techniques offers insights into its therapeutic potential, paving the way for novel strategies in disease intervention, particularly for conditions where angiogenesis is disrupted. The generation of ANG3 recombinant protein facilitates detailed biochemical characterization and functional assays, enabling scientists to elucidate its precise mechanisms of action and role in disease progression. As such, ANG3 represents a promising target in the ongoing quest for effective therapies that harness the body’s natural angiogenic and protective responses.
