Analytical Data
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基因名
CHRNa1
- Application
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别名
CHRNa1；ACHRA；CHNRA；Acetylcholine receptor subunit alpha
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P02708
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表达区间
21-255aa
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氨基酸序列
SEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIV TTNVRLKQGDMVDLPRPSCVTLGVPLFSHLQNEQWVDYNLKWNPDDYGGV KKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTPPAIFK SYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGE WVIKESRGWKHSVTYSCCPDTPYLDITYHFVMQRL
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分子量
44 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cholinergic receptors, specifically the nicotinic acetylcholine receptors (nAChRs), play a crucial role in synaptic transmission and are involved in various physiological functions such as muscle contraction, cognitive processes, and modulation of neurotransmitter release. Among them, the α1 subunit of the nAChR (CHRNa1) has garnered significant interest due to its ubiquitous expression in the nervous system and its implication in various neurological disorders. Research on CHRNa1 recombinant proteins focuses on understanding their biophysical properties, pharmacological profiles, and interaction with ligands. This is particularly important for drug development targeting conditions such as Alzheimer's disease, Parkinson's disease, and nicotine addiction. Advances in recombinant DNA technology have enabled the production and purification of CHRNa1 proteins, allowing for detailed studies of their structure-function relationships. Additionally, these recombinant proteins serve as valuable tools for high-throughput screening of novel compounds and for elucidating the mechanisms underlying receptor activation and signaling pathways. Overall, the study of CHRNa1 recombinant proteins represents a vital component in the broader field of neuropharmacology, aiming to provide insights into receptor behavior and facilitate the development of targeted therapies for cholinergic dysfunctions.
