Analytical Data
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Gene name
GUSb
- Application
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Alternative Names
GUSb；Beta-glucuronidase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P08236
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Expression Region
23-651aa
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AA Sequence
LQGGMLYPQESPSRECKELDGLWSFRADFSDNRRRGFEEQWYRRPLWESG PTVDMPVPSSFNDISQDWRLRHFVGWVWYEREVILPERWTQDLRTRVVLR IGSAHSYAIVWVNGVDTLEHEGGYLPFEADISNLVQVGPLPSRLRITIAI NNTLTPTTLPPGTIQYLTDTSKYPKGYFVQNTYFDFFNYAGLQRSVLLYT TPTTYIDDITVTTSVEQDSGLVNYQISVKGSNLFKLEVRLLDAENKVVAN GTGTQGQLKVPGVSLWWPYLMHERPAYLYSLEVQLTAQTSLGPVSDFYTL PVGIRTVAVTKSQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNL LRWLGANAFRTSHYPYAEEVMQMCDRYGIVVIDECPGVGLALPQFFNNVS LHHHMQVMEEVVRRDKNHPAVVMWSVANEPASHLESAGYYLKMVIAHTKS LDPSRPVTFVSNSNYAADKGAPYVDVICLNSYYSWYHDYGHLELIQLQLA TQFENWYKKYQKPIIQSEYGAETIAGFHQDPPLMFTEEYQKSLLEQYHLG LDQKRRKYVVGELIWNFADFMTEQSPTRVLGNKKGIFTRQRQPKSAAFLL RERYWKIANETRYPHSVAKSQCLENSLFTHHHHHH
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Molecular Weight
73 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GUSb, which stands for β-glucuronidase B, is a variant of the glycosyl hydrolase enzyme that has garnered significant attention in both basic and applied research fields. Originally derived from various sources, such as bacteria and plants, GUSb is known for its ability to catalyze the hydrolysis of glucuronides, thereby playing a crucial role in metabolic processes and biotransformation. Its significance extends to environmental microbiology, where it is utilized as a biomarker for microbial activity and as an indicator for soil health. In the context of genetic engineering and synthetic biology, GUSb serves as a reporter gene, facilitating the monitoring of gene expression and the effectiveness of transformation processes in plant and animal models. Recent studies have focused on optimizing GUSb for enhanced stability and activity, which hold potential implications in biopharmaceuticals and biotechnology applications. The exploration of GUSb's structure-function relationships through molecular biology techniques has provided insights into its catalytic mechanisms, paving the way for innovations in enzyme engineering and therapeutic interventions. Overall, GUSb represents a valuable tool in both academic research and industrial applications, driving advancements in biotechnology, agriculture, and environmental science.
