Analytical Data
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基因名
HARS
- Application
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别名
HARS1;HARS;HRS;Histidine--tRNA ligase. cytoplasmic
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P12081
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表达区间
1-509aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSMAERAALEELVKLQGERVRGLKQQKAS AELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVF DVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSL RYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFD IAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVS DSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGV SLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARG LDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKV PCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKL VSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIK LRSVTSREEVDVRREDLVEEIKRRTGQPLCIC
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分子量
60 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HARS, or Histidyl-tRNA synthetase, is an essential enzyme that plays a critical role in protein synthesis by catalyzing the attachment of histidine to its corresponding tRNA. Its significance in cellular functions is underscored by its involvement in various physiological and pathological processes. Research into HARS has garnered attention due to its implications in various diseases, including neurodegenerative disorders and cancers, where its expression levels and functional integrity may be disrupted. Additionally, HARS is implicated in the regulation of aminoacyl-t RNA synthetases, and its role in the 'unfolded protein response' highlights its connection to cellular stress responses. In recent years, advances in structural biology techniques, such as X-ray crystallography and cryo-electron microscopy, have provided deeper insights into the enzyme's structure-function relationship, contributing to our understanding of its catalytic mechanisms and interactions with other cellular components. Furthermore, HARS research has opened potential avenues for therapeutic interventions, as modulators of its activity could serve as novel strategies for treating diseases associated with its dysregulation. Overall, the study of HARS and its recombinant proteins continues to be a promising field that combines molecular biology, biochemistry, and medicine, with the potential to unlock new understanding of translational control and its impact on human health.
