Analytical Data
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Gene name
ERp57
- Application
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Alternative Names
ERp57；ERP57；ERP60；GRP58；Protein disulfide-isomerase A3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P30101
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Expression Region
25-505aa
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AA Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL
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Molecular Weight
59 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ERp57, also known as endoplasmic reticulum protein 57, is a critical member of the protein disulfide isomerase (PDI) family, primarily involved in the formation and rearrangement of disulfide bonds in nascent glycoproteins within the endoplasmic reticulum. This enzyme plays a pivotal role in protein folding, quality control, and the overall maturation of proteins that are essential for various physiological processes. Studies have highlighted ERp57's involvement in immune responses and its interaction with various chaperones, which influences both protein stability and functionality. Research has also suggested that dysregulation of ERp57 can lead to various diseases, including neurodegenerative disorders and cancers. Therefore, the understanding of ERp57's mechanisms and its interactions has garnered significant interest in the fields of cell biology and molecular medicine. As a potential therapeutic target, recombinant ERp57 has been studied for its capability to improve protein folding and stability, which opens avenues for biotechnological applications in developing biopharmaceuticals. The exploration of ERp57's structure-function relationship through recombinant protein techniques aims to shed light on its role in cellular processes and therapeutic potential, highlighting the importance of this enzyme in both fundamental research and practical applications in disease treatment and biotechnology.
