Analytical Data
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Gene name
KCNK18
- Application
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Alternative Names
KCNK18; TRESK; TRIK; Potassium channel subfamily K member 18; TWIK-related individual potassium channel; TWIK-related spinal cord potassium channel
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z418
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Expression Region
1-384aa
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AA Sequence
MEVSGHPQARRCCPEALGKLFPGLCFLCFLVTYALVGAVVFSAIEDGQVLVAADDGEFEKFLEELCRILNCSETVVEDRKQDLQGHLQKVKPQWFNRTTHWSFLSSLFFCCTVFSTVGYGYIYPVTRLGKYLCMLYALFGIPLMFLVLTDTGDILATILSTSYNRFRKFPFFTRPLLSKWCPKSLFKKKPDPKPADEAVPQIIISAEELPGPKLGTCPSRPSCSMELFERSHALEKQNTLQLPPQAMERSNSCPELVLGRLSYSIISNLDEVGQQVERLDIPLPIIALIVFAYISCAAAILPFWETQLDFENAFYFCFVTLTTIGFGDTVLEHPNFFLFFSIYIIVGMEIVFIAFKLVQNRLIDIYKNVMLFFAKGKFYHLVKK
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Molecular Weight
69.19 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KCNK18, also known as the K2P18.1 channel, is a member of the two-pore domain potassium channel family, which plays a crucial role in maintaining the resting membrane potential and regulating neuronal excitability. This channel is of particular interest due to its unique voltage and pH sensitivity characteristics, making it a potential candidate for novel therapeutic targets in the treatment of various neurological disorders and cardiovascular conditions. Research into KCNK18 has revealed its involvement in mechanisms such as ion homeostasis and cell signaling, which are vital for cellular functioning. Given its expression in the nervous system and potential implications in pain sensation and respiratory response, studies are increasingly focused on understanding its channel properties, subcellular localization, and biological functions. The production of recombinant KCNK18 proteins allows for detailed biophysical and pharmacological studies, providing insights into channel gating mechanisms and interactions with potential pharmacological agents. Such investigations could pave the way for the development of drugs aimed at modulating KCNK18 activity, thus offering new avenues for addressing diseases linked to potassium channel dysfunction. Understanding KCNK18 further enriches our knowledge of potassium channels and their role in health and disease, highlighting the significance of this research in the broader context of ion channel biology and therapeutic innovation.
