Cat: PA2000-392DB

Recombinant Human DHEA Protein,His

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Analytical Data

  • Gene name

    DHEA

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    DHEA;HST;STD;Sulfotransferase 2A1

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P43403

  • Expression Region

    1-619aa

  • AA Sequence

    MPDPAAHLPF FYGSISRAEA EEHLKLAGMA DGLFLLRQCL RSLGGYVLSL VHDVRFHHFP IERQLNGTYA IAGGKAHCGP AELCEFYSRD PDGLPCNLRK PCNRPSGLEP QPGVFDCLRD AMVRDYVRQT WKLEGEALEQ AIISQAPQVE KLIATTAHER MPWYHSSLTR EEAERKLYSG AQTDGKFLLR PRKEQGTYAL SLIYGKTVYH YLISQDKAGK YCIPEGTKFD TLWQLVEYLK LKADGLIYCL KEACPNSSAS NASGAAAPTL PAHPSTLTHP QRRIDTLNSD GYTPEPARIT SPDKPRPMPM DTSVYESPYS DPEELKDKKL FLKRDNLLIA DIELGCGNFG SVRQGVYRMR KKQIDVAIKV LKQGTEKADT EEMMREAQIM HQLDNPYIVR LIGVCQAEAL MLVMEMAGGG PLHKFLVGKR EEIPVSNVAE LLHQVSMGMK YLEEKNFVHR DLAARNVLLV NRHYAKISDF GLSKALGADD SYYTARSAGK WPLKWYAPEC INFRKFSSRS DVWSYGVTMW EALSYGQKPY KKMKGPEVMA FIEQGKRMEC PPECPPELYA LMSDCWIYKW EDRPDFLTVE QRMRACYYSL ASKVEGPPGS TQKAEAACA

  • Molecular Weight

    69.8 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

Dehydroepiandrosterone (DHEA) is an endogenous steroid hormone primarily produced by the adrenal glands, with a crucial role in the biosynthesis of androgens and estrogens. Research into recombinant DHEA protein has gained momentum due to its potential therapeutic applications. DHEA levels peak in early adulthood and decline with age, influencing various physiological processes, including immune function, bone density, and metabolism. Studies suggest that restoring DHEA levels may alleviate age-related conditions, improve mood, and enhance cognitive function. However, the challenge lies in the complexity of its metabolism and the varying effects of supplemental DHEA. The advent of recombinant DNA technology allows for the production of engineered DHEA proteins, providing opportunities to explore structure-function relationships, pharmacokinetics, and efficacy in clinical settings. This approach not only aims to overcome limitations associated with traditional hormone replacement therapies but also facilitates the development of targeted treatments that maximize benefits while minimizing side effects. Consequently, the ongoing research into recombinant DHEA protein holds promise for advancing our understanding of hormonal regulation and its implications for aging and healthspan enhancement.

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