Analytical Data
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基因名
KDM6B
- Application
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别名
KDM6B；JMJD3；KIAA0346；Lysine-specific demethylase 6B
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15054
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表达区间
1176-1505aa
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氨基酸序列
LPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEV
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分子量
41.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KDM6B, also known as lysine demethylase 6B, is a member of the JmjC domain-containing family of proteins, which are implicated in the regulation of gene expression through histone modification. This enzyme specifically demethylates tri- and di-methylated lysine 27 on histone H3 (H3K27me3/2), a modification associated with transcriptional repression and chromatin compaction. The role of KDM6B in epigenetic regulation has drawn significant attention in recent years due to its potential implications in various biological processes, including development, differentiation, and cellular response to environmental stimuli. Furthermore, dysregulation of KDM6B has been linked to several diseases, particularly cancers, where its altered expression can contribute to oncogenesis by reactivating silenced tumor suppressor genes. The study of KDM6B recombinant proteins has enabled researchers to elucidate its biochemical properties, interaction partners, and functional mechanisms. Understanding how KDM6B influences chromatin dynamics and gene expression is crucial for developing potential therapeutic strategies that target epigenetic modifications in disease contexts. Through the exploration of KDM6B's enzymatic activity and regulatory pathways, researchers aim to better comprehend its role in cellular homeostasis and disease progression, paving the way for novel interventions in cancer and other pathologies associated with epigenetic dysregulation.
