Analytical Data
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Gene name
TSC1
- Application
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Alternative Names
TSC1；KIAA0243；TSC；Hamartin
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q92574
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Expression Region
690-993aa
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AA Sequence
TLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYNQLQEQRDTMVTKLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQTQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERL
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Molecular Weight
42.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TSC1 (Tuberous Sclerosis Complex 1) is a critical gene involved in the regulation of cell growth and proliferation, and it plays a vital role in the Tuberous Sclerosis Complex (TSC), a genetic disorder characterized by the development of benign tumors in various organs, including the brain, kidneys, and skin. The TSC1 protein, also known as hamartin, interacts with TSC2 (tuberin) to form a heterodimer that regulates the mTOR (mammalian target of rapamycin) signaling pathway, which is crucial for cellular metabolism, growth, and autophagy. Mutations in TSC1 or TSC2 can lead to dysregulation of the mTOR pathway, contributing to tumorigenesis and a range of neurological symptoms. Given the significance of TSC1 in maintaining cellular homeostasis and its implications in cancer biology, researchers are increasingly focused on the production and characterization of recombinant TSC1 protein. This research not only aims to elucidate the molecular mechanisms governing TSC pathology but also holds potential for therapeutic interventions, such as targeted mTOR inhibitors. Investigating the structure and function of TSC1 can provide insights into its role in cellular signaling and disease progression, thus paving the way for the development of novel treatment strategies for TSC and related disorders. The availability of functional recombinant TSC1 protein is essential for these endeavors, enabling biochemical assays and structural studies that enrich our understanding of this complex disease at the molecular level.
