Analytical Data
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基因名
MAPK3
- Application
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别名
MAPKAPK3;MAP kinase-activated Protein kinase 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P27361
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表达区间
2-379aa
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氨基酸序列
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAAAAQGGGGGEPRRTEGV GPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAI KKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYI VQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS NLLSNTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSK GYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLN CIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRIT VEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETAR FQPGVLEAP
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分子量
70 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Mitogen-activated protein kinase 3 (MAPK3), also known as extracellular signal-regulated kinase 1 (ERK1), is a critical component of the MAPK signaling pathway, which regulates various cellular processes including proliferation, differentiation, and survival in response to extracellular stimuli. The MAPK pathway is essential for translating environmental signals into cellular responses, and its dysregulation is implicated in numerous diseases, particularly cancer. Research into MAPK3 has gained prominence due to its role in mediating the effects of growth factors and other mitogens on cell function. Recombinant MAPK3 protein has been utilized extensively in both in vitro and in vivo studies to elucidate its biological functions and intricate regulatory mechanisms. Furthermore, recombinant MAPK3 has been instrumental in developing novel therapeutic strategies targeting MAPK pathways, with a focus on inhibiting aberrant signaling in cancer cells. The expression and purification of active recombinant MAPK3 allow researchers to perform kinetic analyses and screen for potential inhibitors, paving the way for new treatments. Additionally, understanding the post-translational modifications and interactions of MAPK3 can reveal critical insights into its role in cellular signaling networks. Given its central role in key physiological processes and its involvement in various pathologies, MAPK3 remains a compelling target for biomedical research and drug development.
