Analytical Data
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Gene name
pks13
- Application
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Alternative Names
pks13；Polyketide synthase Pks13
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8RWC9
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Expression Region
1-444aa
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AA Sequence
MVRRQEEEKK AEKGMRLGKY ELGRTLGEGN FGKVKFAKDT VSGHSFAVKI IDKSRIADLN FSLQIKREIR TLKMLKHPHI VRLHEVLASK TKINMVMELV TGGELFDRIV SNGKLTETDG RKMFQQLIDG ISYCHSKGVF HRDLKLENVL LDAKGHIKIT DFGLSALPQH FRDDGLLHTT CGSPNYVAPE VLANRGYDGA ASDIWSCGVI LYVILTGCLP FDDRNLAVLY QKICKGDPPI PRWLSPGART MIKRMLDPNP VTRITVVGIK ASEWFKLEYI PSIPDDDDEE EVDTDDDAFS IQELGSEEGK GSDSPTIINA FQLIGMSSFL DLSGFFEQEN VSERRIRFTS NSSAKDLLEK IETAVTEMGF SVQKKHAKLR VKQEERNQKG QVGLSVTAEV FEIKPSLNVV ELRKSYGDSC LYRQLYERLL KDVGTSSPEQ EIVT
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Molecular Weight
49.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PKS13 (polyketide synthase 13) is a crucial enzyme involved in the biosynthesis of lipoic acid, a vital cofactor necessary for various cellular processes, particularly in bacteria and eukaryotic organisms. The study of PKS13 is important due to its role in the pathogenicity of Mycobacterium tuberculosis, the bacterium responsible for tuberculosis. Understanding the function and structure of PKS13 can provide insights into its mechanistic pathways and potential regulatory mechanisms. Recent advancements in molecular biology techniques, including gene cloning, expression systems, and protein purification, have facilitated detailed investigations into PKS13’s activity and interactions. Additionally, as antibiotic resistance becomes an increasing threat, targeting enzymes like PKS13 presents a promising avenue for developing novel therapeutic strategies. Research has highlighted the potential of PKS13 inhibitors to serve as antimicrobial agents, which could be particularly beneficial in treating drug-resistant strains of tuberculosis. By elucidating the biochemical pathways and structural characteristics of PKS13, scientists aim to better understand its functionality and explore its role in antibiotic biosynthesis. This research not only contributes to the fundamental knowledge of microbial metabolism but also paves the way for innovative drug design initiatives aimed at combating tuberculosis and other infections linked to PKS13. Thus, PKS13 remains a significant focus of research within the fields of microbiology, biochemistry, and pharmaceutical sciences, reflecting the urgent need for new approaches in infectious disease management.
