Analytical Data
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Gene name
LEPRE1
- Application
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Alternative Names
GROS 1; GROS1; Growth suppressor 1; LEPRE 1; Lepre1; Leprecan 1; Leprecan; Leprecan-1; Leprecan1; Leucine proline enriched proteoglycan (leprecan) 1; Leucine proline enriched proteoglycan 1; Leucine- and proline-enriched proteoglycan 1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q32P28
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Expression Region
23-736aa
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AA Sequence
EVESEAGW GMVTPDLLFA EGTAAYARGD WPGVVLSMER ALRSRAALRA LRLRCRTQCA ADFPWELDPD WSPSPAQASG AAALRDLSFF GGLLRRAACL RRCLGPPAAH SLSEEMELEF RKRSPYNYLQ VAYFKINKLE KAVAAAHTFF VGNPEHMEMQ QNLDYYQTMS GVKEADFKDL ETQPHMQEFR LGVRLYSEEQ PQEAVPHLEA ALQEYFVAYE ECRALCEGPY DYDGYNYLEY NADLFQAITD HYIQVLNCKQ NCVTELASHP SREKPFEDFL PSHYNYLQFA YYNIGNYTQA VECAKTYLLF FPNDEVMNQN LAYYAAMLGE EHTRSIGPRE SAKEYRQRSL LEKELLFFAY DVFGIPFVDP DSWTPEEVIP KRLQEKQKSE RETAVRISQE IGNLMKEIET LVEEKTKESL DVSRLTREGG PLLYEGISLT MNSKLLNGSQ RVVMDGVISD HECQELQRLT NVAATSGDGY RGQTSPHTPN EKFYGVTVFK ALKLGQEGKV PLQSAHLYYN VTEKVRRIME SYFRLDTPLY FSYSHLVCRT AIEEVQAERK DDSHPVHVDN CILNAETLVC VKEPPAYTFR DYSAILYLNG DFDGGNFYFT ELDAKTVTAE VQPQCGRAVG FSSGTENPHG VKAVTRGQRC AIALWFTLDP RHSERDRVQA DDLVKMLFSP EEMDLSQEQP LDAQQGPPEP AQESLSGSES KPKDEL
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Molecular Weight
83.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LEPRE1, or leprecan-like 1, is a protein that plays a crucial role in collagen biosynthesis and modification. Its primary function involves the post-translational modification of collagen, specifically in the hydroxylation of proline residues, which is essential for the stability and structural integrity of collagen fibers. Mutations in the LEPRE1 gene have been linked to various connective tissue disorders, including cartilage-hair hypoplasia and some forms of osteogenesis imperfecta. These conditions often manifest as skeletal abnormalities, emphasizing the importance of LEPRE1 in normal bone and cartilage function. Recent studies have focused on the recombinant expression of LEPRE1 to better understand its functional properties and its role in collagen synthesis. By producing a recombinant version of this protein, researchers aim to dissect its molecular mechanisms, explore its interactions with other collagen-modifying enzymes, and investigate potential therapeutic applications. This line of research holds promise for the development of novel treatments that could mitigate the effects of connective tissue disorders stemming from LEPRE1 mutations, ultimately improving the quality of life for affected individuals. Through structural characterization and functional assays, the recombinant LEPRE1 protein could provide insights into the biochemical pathways involved in collagen maturation and its implications for regenerative medicine and tissue engineering.
