Analytical Data
-
基因名
NDUFA4
- Application
-
别名
NDUFA4;Cytochrome c oxidase subunit NDUFA4
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
O00483
-
表达区间
38-81aa
-
氨基酸序列
LFNPDVCWDRNNPEPWNKLGPNDQYKFYSVNVDYSKLKKERPDF
-
分子量
32.1 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NDUFA4, or NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 4, is a critical component of the mitochondrial respiratory chain, specifically involved in complex I, which is pivotal for ATP production through oxidative phosphorylation. Research into NDUFA4 has gained prominence due to its potential implications in various diseases, including neurodegenerative disorders and certain types of cancer. The protein's role in maintaining mitochondrial function and its involvement in cellular energy metabolism underscore its significance in health and disease. Moreover, NDUFA4 has been shown to interact with other protein complexes and may influence the balance between cell survival and apoptosis, making it a target of interest for therapeutic interventions. The recombinant production of NDUFA4 enables detailed structural and functional studies, allowing researchers to investigate its precise mechanism of action and its interactions within the respiratory chain. Understanding these aspects could provide insights into the pathogenic mechanisms underlying mitochondrial dysfunction and aid in the development of targeted therapies for diseases associated with impaired energy metabolism. The ongoing research aims to elucidate the role of NDUFA4 not only in mitochondrial bioenergetics but also in broader cellular contexts, thereby expanding our knowledge of its biological importance.
