Analytical Data
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Gene name
C3 Convertase
- Application
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Alternative Names
C3 Convertase；BF；BFD；Complement factor B
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P27918
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Expression Region
28-469aa
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AA Sequence
DPV LCFTQYEESS GKCKGLLGGG VSVEDCCLNT AFAYQKRSGG LCQPCRSPRW SLWSTWAPCS VTCSEGSQLR YRRCVGWNGQ CSGKVAPGTL EWQLQACEDQ QCCPEMGGWS GWGPWEPCSV TCSKGTRTRR RACNHPAPKC GGHCPGQAQE SEACDTQQVC PTHGAWATWG PWTPCSASCH GGPHEPKETR SRKCSAPEPS QKPPGKPCPG LAYEQRRCTG LPPCPVAGGW GPWGPVSPCP VTCGLGQTME QRTCNHPVPQ HGGPFCAGDA TRTHICNTAV PCPVDGEWDS WGEWSPCIRR NMKSISCQEI PGQQSRGRTC RGRKFDGHRC AGQQQDIRHC YSIQHCPLKG SWSEWSTWGL CMPPCGPNPT RARQRLCTPL LPKYPPTVSM VEGQGEKNVT FWGRPLPRCE ELQGQKLVVE EKRPCLHVPA CKDPEEEEL
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Molecular Weight
51.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
C3 convertase is a crucial enzyme in the complement system, playing a pivotal role in immune responses by facilitating the cleavage of complement component C3 into C3a and C3b. This process is fundamental for opsonization, inflammation, and the formation of the membrane attack complex. Research on C3 convertase has gained significant attention due to its implications in various diseases, including autoimmune disorders, infections, and inflammatory conditions. Understanding its structure and function is essential for developing targeted therapies and diagnostics. Recombinant forms of C3 convertase have been generated for detailed biochemical studies, allowing researchers to investigate its catalytic mechanisms, inhibitor interactions, and regulatory pathways. Furthermore, these studies contribute to the broader understanding of the complement system's role in health and disease, paving the way for novel therapeutic strategies aimed at modulating complement activity. The advancement of recombinant protein technology has enabled the production of C3 convertase in manageable quantities, facilitating in-depth exploration of its potential as a therapeutic target or biomarker in clinical settings. Overall, the study of C3 convertase not only enhances our understanding of immune mechanisms but also holds promise for innovative treatments for complement-related diseases.
