Analytical Data
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Gene name
DFFB
- Application
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Alternative Names
DFFB；CAD；DFF2；DFF40；DNA fragmentation factor subunit beta
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O76075
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Expression Region
1-338aa
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AA Sequence
MLQKPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQGYVSDIRRFLSAFHEPQVGLIQAAQQLLCDEQAPQRQRLLADLLHNVSQNIAAETRAEDPPWFEGLESRFQSKSGYLRYSCESRIRSYLREVSSYPSTVGAEAQEEFLRVLGSMCQRLRSMQYNGSYFDRGAKGGSRLCTPEGWFSCQGPFDMDSCLSRHSINPYSNRESRILFSTWNLDHIIEKKRTIIPTLVEAIKEQDGREVDWEYFYGLLFTSENLKLVHIVCHKKTTHKLNCDPSRIYKPQTRLKRKQPVRKRQ
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Molecular Weight
45.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DFFB, or DNA Fragmentation Factor Subunit Beta, is a crucial protein involved in the apoptotic process, particularly in the execution phase of programmed cell death. The regulation of apoptosis is essential for maintaining cellular homeostasis and tissue development, and abnormalities in these processes can lead to various diseases, including cancer. The DFFB protein forms a heterodimer with its counterpart, DFFA (DFF Subunit A), and together they facilitate the cleavage of DNA at internucleosomal sites, leading to the characteristic DNA laddering observed in apoptotic cells. Research on DFFB has gained significant attention due to its potential as a therapeutic target; modulating its activity could influence cell survival and death, offering strategies for treating disorders related to dysregulated apoptosis. Additionally, understanding the molecular mechanisms governing DFFB's function and its interactions with other cellular factors is vital for elucidating the broader apoptotic signaling pathways. Recent studies have employed various recombinant DNA techniques to produce and characterize DFFB, providing insights into its structure-function relationships and the impact of post-translational modifications on its activity. As a result, the investigation of DFFB not only enhances our understanding of cell death mechanisms but also opens new avenues for drug development aimed at manipulating apoptotic pathways for therapeutic benefit.
