Analytical Data
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基因名
SPT14
- Application
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别名
SPT14；CWH6；GPI3；Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P32363
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表达区间
1-377aa
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氨基酸序列
MGFNIAMLCDFFYPQLGGVEFHIYHLSQKLIDLGHSVVIITHAYKDRVGVRHLTNGLKVYHVPFFVIFRETTFPTVFSTFPIIRNILLREQIQIVHSHGSASTFAHEGILHANTMGLRTVFTDHSLYGFNNLTSIWVNKLLTFTLTNIDRVICVSNTCKENMIVRTELSPDIISVIPNAVVSEDFKPRDPTGGTKRKQSRDKIVIVVIGRLFPNKGSDLLTRIIPKVCSSHEDVEFIVAGDGPKFIDFQQMIESHRLQKRVQLLGSVPHEKVRDVLCQGDIYLHASLTEAFGTILVEAASCNLLIVTTQVGGIPEVLPNEMTVYAEQTSVSDLVQATNKAINIIRSKALDTSSFHDSVSKMYDWMDVAKRTVEIYTN
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分子量
49.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SPT14, also known as Suppressor of Ty 14, is a protein that plays a critical role in various cellular processes, including transcriptional regulation and stress response. Research on SPT14 has gained significant attention due to its involvement in chromatin remodeling and gene expression. Specifically, studies have shown that SPT14 is a key component of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, which is essential for histone acetylation and transcriptional activation. The understanding of SPT14's function has implications in developmental biology, cancer research, and the study of hereditary diseases, where dysregulation of gene expression can lead to pathological conditions. Recent advances in recombinant protein technology have enabled the production and purification of SPT14, facilitating in-depth biochemical characterization and functional assays. By examining how SPT14 interacts with other components of the transcription machinery and understanding its structural features, researchers aim to elucidate its precise role in cellular homeostasis. The insights gained from SPT14 studies could pave the way for novel therapeutic strategies targeting gene regulation and transcriptional misfires in various diseases, making it a focal point in contemporary molecular biology research.
