Analytical Data
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基因名
ACTA1
- Application
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别名
ACTA1；ACTA；Actin. alpha skeletal muscle
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P68133
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表达区间
3-377aa
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氨基酸序列
DEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF
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分子量
47.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACTA1, encoding skeletal muscle alpha-actin, plays a crucial role in muscle contraction and structure. Mutations in ACTA1 are associated with various myopathies, highlighting its importance in muscle biology. The study of ACTA1 recombinant protein is essential for understanding the underlying mechanisms of these muscle disorders. Researchers have been focused on producing and characterizing recombinant ACTA1 to elucidate its folding, stability, and interactions with other proteins. By using techniques such as molecular cloning, expression in prokaryotic and eukaryotic systems, and functional assays, scientists aim to investigate the biochemical properties of ACTA1 and its impact on muscle function. This research is particularly relevant for developing potential therapeutic strategies for ACTA1-related myopathies. The recombinant protein serves not only as a valuable tool for structural and functional studies but also as a platform for screening small molecules that might correct the defects associated with the mutated protein. Overall, ACTA1 recombinant protein studies contribute significantly to the understanding of skeletal muscle physiology and pathology.
