Analytical Data
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基因名
MDH1B
- Application
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别名
MDH1B; Putative malate dehydrogenase 1B; EC 1.1.1.-
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q5I0G3
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表达区间
1-518aa
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氨基酸序列
MAKFVIAGRA DCPYYAKTEL VADYLQKNLP DFRIHKITQR PEVWEDWLKD VCEKNKWSHK NSPIIWRELL DRGGKGLLLG GYNEFLEHAQ LYYDVTSSMT TELMMVIAQE NLGAHIEKEQ EEEALKTCIN PLQVWITSAS APACYNLIPI LTSGEVFGMH TEISITLFDN KQAEEHLKSL VVETQDLASP VLRSVSICTK VEEAFRQAHV IVVLDDSTNK EVFTLEDCLR SRVPLCRLYG YLIEKNAHES VRVIVGGRTF VNLKTVLLMR YAPRIAHNII AVALGVEGEA KAILARKLKT APSYIKDVII WGNISGNNYV DLRKTRVYRY ESAIWGPLHY SRPVLNLIFD SEWVKREFVA ILKNLTTTGR QFGGILAAHS IATTLKYWYH GSPPGEIVSL GILSEGQFGI PKGIVFSMPV KFENGTWVVL TDLKDVEISE QIMTRMTSDL IQEKLVALGD KIHFQPYQSG HKDLVPDEEK NLAMSDAAEF PNQIPQTTFE KPQSLEFLNE FEGKTVES
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分子量
58,6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MDH1B, or Malate Dehydrogenase 1B, is a key enzyme involved in the citric acid cycle, playing a crucial role in cellular metabolism by catalyzing the reversible conversion of malate to oxaloacetate. Research on MDH1B is gaining momentum due to its potential implications in various physiological and pathological processes, including metabolic disorders, cancer development, and neurodegenerative diseases. Studies have shown that alterations in MDH1B activity can influence cellular respiration and energy production, which are critical in maintaining cellular homeostasis. Furthermore, the enzyme is of interest in the context of plant biology, as it contributes to stress responses and adaptation mechanisms. Recent advances in biotechnology have facilitated the recombinant expression of MDH1B, allowing researchers to obtain large quantities of the protein for in-depth studies. Understanding the structure and function of MDH1B through recombinant protein techniques not only enhances our comprehension of its role in metabolism but also aids in the identification of potential therapeutic targets. The exploration of MDH1B's physiological functions and its involvement in disease mechanisms presents promising avenues for the development of novel treatments, further underscoring the importance of this enzyme in both health and disease.
