Analytical Data
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基因名
MGAT5B
- Application
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别名
MGAT5B; KIAA2008Alpha-1.6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B; EC 2.4.1.-; EC 2.4.1.155; Alpha-mannoside beta-1.6-N-acetylglucosaminyltransferase B; GlcNAc-T Vb; GNT-Vb; hGnTVb; Mannoside acetylglucosaminyltransferase 5B; N-acetylglucosaminyl-transferase Vb; N-acetylglucosaminyltransferase IX; GNT-IX
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q3V5L5
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表达区间
1-394 aa
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氨基酸序列
MITVNPDGKIMVRRCLVTLRPFRLFVLGIGFFTLCFLMTSLGGQFSARRLGDSPFTIRTEVMGGPESRGVLRKMSDLLELMVKRMDALARLENSSELHRAGGDLHFPADRMPPGAGLMERIQAIAQNVSDIAVKVDQILRHSLLLHSKVSEGRRDQCEAPSDPKFPDCSGKVEWMRARWTSDPCYAFFGVDGTECSFLIYLSEVEWFCPPLPWRNQTAAQRAPKPLPKVQAVFRSNLSHLLDLMGSGKESLIFMKKRTKRLTAQWALAAQRLAQKLGATQRDQKQILVHIGFLTEESGDVFSPRVLKGGPLGEMVQWADILTALYVLGHGLRVTVSLKELQRQRRLRSSQEQARCGVMGACEKMLLEGDVASNTLLIEKPSKKETEKCPKNLVT
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分子量
70.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MGAT5B, a member of the MGAT (mannosyltransferase) family, plays a crucial role in glycosylation, specifically in the synthesis of complex N-glycans. This enzyme is known for its ability to catalyze the addition of mannose residues to glycoproteins, which is essential for proper protein folding, stability, and function. Alterations in MGAT5B activity have been linked to various pathological conditions, including cancer, where changes in glycosylation patterns can influence tumor progression and immune response. The study of recombinant MGAT5B protein is vital for understanding its enzymatic function and the implications of its activity in disease. By producing this protein through recombinant techniques, researchers can analyze its biochemical properties, investigate its role in N-glycan biosynthesis, and explore potential therapeutic applications. Additionally, recombinant MGAT5B can serve as a valuable tool for studying glycoprotein modifications and their effects on cellular interactions and signaling pathways, thereby providing insights into the critical impacts of glycosylation in biology and medicine. As such, the exploration of MGAT5B through recombinant protein technology continues to be a significant area of interest within glycobiology and functional genomics.
