Analytical Data
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Gene name
PKCK2
- Application
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Alternative Names
;
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16822
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Expression Region
1-640aa
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AA Sequence
MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEH SARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPK DVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPG CMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQ ALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFG SGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAA AFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGF FGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVT VTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAII FGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAM RPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGE NARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPK DFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM
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Molecular Weight
96 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PKCK2 (protein kinase CK2) is a serine/threonine kinase that plays a pivotal role in various cellular processes, including cell proliferation, differentiation, and apoptosis. Its dysregulation has been implicated in numerous diseases, particularly cancer, making it a target of considerable interest in therapeutic development. With its ubiquitous expression and multifaceted functions, PKCK2 is involved in various signaling pathways, modulating the activity of many substrates by phosphorylating key amino acid residues. The research on recombinant PKCK2 proteins focuses on understanding the enzyme's structure-function relationship, regulatory mechanisms, and substrate interactions. By producing PKCK2 in a recombinant system, researchers can obtain pure protein for detailed biochemical characterization, high-throughput screening of inhibitors, and functional assays. Structural studies employing techniques such as X-ray crystallography and NMR spectroscopy are instrumental in revealing the conformation of PKCK2 and its complexes with substrates and inhibitors, which can guide drug design efforts. Additionally, the study of PKCK2's role in oncogenic processes, including its influence on transcription factors and signaling molecules, provides insights into its potential as a biomarker for cancer prognosis and response to therapy. Overall, the exploration of recombinant PKCK2 proteins is crucial for elucidating its biological significance and advancing our understanding of its contributions to disease, thereby paving the way for novel therapeutic strategies targeting PKCK2.
