Analytical Data
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基因名
PUF60
- Application
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别名
PUF60；FIR；ROBPI；SIAHBP1；Poly(U)-binding-splicing factor PUF60
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UHX1
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表达区间
1-559aa
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氨基酸序列
MATATIALQV NGQQGGGSEP AAAAAVVAAG DKWKPPQGTD SIKMENGQST AAKLGLPPLT PEQQEALQKA KKYAMEQSIK SVLVKQTIAH QQQQLTNLQM AAVTMGFGDP LSPLQSMAAQ RQRALAIMCR VYVGSIYYEL GEDTIRQAFA PFGPIKSIDM SWDSVTMKHK GFAFVEYEVP EAAQLALEQM NSVMLGGRNI KVGRPSNIGQ AQPIIDQLAE EARAFNRIYV ASVHQDLSDD DIKSVFEAFG KIKSCTLARD PTTGKHKGYG FIEYEKAQSS QDAVSSMNLF DLGGQYLRVG KAVTPPMPLL TPATPGGLPP AAAVAAAAAT AKITAQEAVA GAAVLGTLGT PGLVSPALTL AQPLGTLPQA VMAAQAPGVI TGVTPARPPI PVTIPSVGVV NPILASPPTL GLLEPKKEKE EEELFPESER PEMLSEQEHM SISGSSARHM VMQKLLRKQE STVMVLRNMV DPKDIDDDLE GEVTEECGKF GAVNRVIIYQ EKQGEEEDAE IIVKIFVEFS IASETHKAIQ ALNGRWFAGR KVVAEVYDQE RFDNSDLSA
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分子量
59.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PUF60, a member of the PUF (Pumilio and FBF) family of RNA-binding proteins, plays a crucial role in the regulation of gene expression at the post-transcriptional level. It is involved in various cellular processes, including RNA stability, splicing, and translation, making it essential for maintaining normal cellular functions. Recent studies have highlighted its significance in several biological contexts, such as embryonic development, cellular differentiation, and response to stress. Dysregulation of PUF60 has been associated with various diseases, including cancer and neurodegenerative disorders, underscoring its potential as a therapeutic target. Research into recombinant PUF60 protein has provided valuable insights into its structure and function, paving the way for understanding its interaction with RNA and other protein partners. By employing techniques such as co-immunoprecipitation and RNA affinity chromatography, scientists aim to elucidate the specific molecular mechanisms governed by PUF60. The study of this protein not only enhances our comprehension of post-transcriptional regulation but also opens avenues for potential interventions in diseases linked to its dysfunction.
