Analytical Data
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Gene name
POLB
- Application
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Alternative Names
POLB;DNA polymerase beta
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06746
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Expression Region
1-335aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMSKRKAPQETLNGGITDMLTELANFEKNVS QAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATG KLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNED KLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGS FRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSK GETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNK NMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREP KDRSE
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Molecular Weight
40 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLB, or Polymerase Beta, is a DNA polymerase that plays a crucial role in the base excision repair (BER) pathway, which is vital for the maintenance of genomic stability by repairing damaged DNA. It is known for its ability to incorporate nucleotides opposite damaged bases and fill in single-strand breaks. The study of POLB has garnered significant attention due to its implications in cancer biology, as dysregulation or mutations in POLB may contribute to tumorigenesis and resistance to certain chemotherapeutic agents. Additionally, POLB has been implicated in neurological disorders, emphasizing its importance beyond cancer. Researchers have been focusing on understanding the structural and functional aspects of POLB through various biochemical and biophysical techniques, including X-ray crystallography and single-molecule analysis, to unravel its mechanism of action and regulation. The recombinant expression of POLB allows for the production of large quantities of the enzyme, facilitating in-depth studies of its catalytic properties and interactions with other repair factors. This research is essential for developing targeted therapies that could enhance the efficacy of existing treatments by modulating POLB activity, potentially leading to novel strategies for combating cancer and other diseases associated with DNA damage. Overall, POLB represents a critical target for understanding DNA repair mechanisms and developing therapeutic innovations in the fight against diseases linked to genomic instability.
