Analytical Data
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基因名
POLR2J
- Application
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别名
AATF;CHE1;DED;Protein AATF
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P52435
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表达区间
1-117aa
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氨基酸序列
MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTNA ITDLISELSL LEERFRVAIK DKQEGIE
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Polymerase II (POLR2J) is a crucial component of the RNA polymerase II complex, responsible for transcribing DNA into mRNA in eukaryotic cells. This complex is essential for gene expression regulation and is involved in various cellular processes, including cell differentiation, response to stress, and development. Dysregulation of RNA polymerase II activity has been implicated in numerous diseases, including cancer and neurodegenerative disorders, making the understanding of its subunits, like POLR2J, particularly important. Research on POLR2J has revealed its role in the modulation of transcriptional dynamics and its interaction with various transcription factors and regulatory proteins. The recombinant expression of POLR2J allows for detailed studies of its structural properties, functional mechanisms, and interaction networks. Furthermore, characterizing POLR2J through recombinant protein techniques can aid in the identification of potential therapeutic targets and enhance our understanding of transcription machinery in health and disease. Given the pivotal role of RNA polymerase II in gene regulation, the functional analysis of POLR2J offers promising insights into the molecular mechanisms governing gene expression, ultimately contributing to advancements in biomedical research and therapeutic development.
