Analytical Data
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基因名
RNF34
- Application
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别名
RNF34;E3 ubiquitin-Protein ligase RNF34
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q969K3
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表达区间
1-372aa
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氨基酸序列
MKAGATSMWASCCGLLNEVMGTGAVRGQQSAFAGATGPFRFTPNPEFSTY PPAATEGPNIVCKACGLSFSVFRKKHVCCDCKKDFCSVCSVLQENLRRCS TCHLLQETAFQRPQLMRLKVKDLRQYLILRNIPIDTCREKEDLVDLVLCH HGLGSEDDMDTSSLNSSRSQTSSFFTRSFFSNYTAPSATMSSFQGELMDG DQTSRSGVPAQVQSEITSANTEDDDDDDDEDDDDEEENAEDRNPGLSKER VRASLSDLSSLDDVEGMSVRQLKEILARNFVNYSGCCEKWELVEKVNRLY KENEENQKSYGERLQLQDEEDDSLCRICMDAVIDCVLLECGHMVTCTKCG KRMSECPICRQYVVRAVHVFKS
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分子量
68 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF34, a member of the RING finger E3 ubiquitin ligase family, plays a crucial role in regulating various cellular processes, including protein degradation, signal transduction, and cell cycle progression. Its involvement in the ubiquitin-proteasome pathway highlights its potential impact on various diseases, including cancer and neurodegenerative disorders. Recent studies have suggested that RNF34 may modulate the stability of key regulatory proteins, influencing pathways associated with cell proliferation and apoptosis. Understanding the molecular mechanisms governing RNF34 function is essential for elucidating its role in disease contexts and may provide novel therapeutic targets. Researchers have focused on developing recombinant RNF34 proteins to study their structure, function, and interactions with other proteins. By employing techniques such as X-ray crystallography and NMR spectroscopy, scientists aim to uncover the intricate details of RNF34’s mechanism of action. Furthermore, the generation of RNF34 knockdown and overexpression models in cell lines facilitates investigations into its biological significance. As the understanding of RNF34 continues to expand, it holds promise for advancing our knowledge of ubiquitin ligases and their therapeutic potential in targeting diseases driven by aberrant protein regulation.
