Analytical Data
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基因名
NEK5
- Application
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别名
NEK5; Serine/threonine-protein kinase Nek5; EC 2.7.11.1; Never in mitosis A-related kinase 5; NimA-related protein kinase 5
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6P3R8
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表达区间
1-708 aa
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氨基酸序列
MDKYDVIKAI GQGAFGKAYL AKGKSDSKHC VIKEINFEKM PIQEKEASKK EVILLEKMKH PNIVAFFNSF QENGRLFIVM EYCDGGDLMK RINRQRGVLF SEDQILGWFV QISLGLKHIH DRKILHRDIK AQNIFLSKNG MVAKLGDFGI ARVLNNSMEL ARTCIGTPYY LSPEICQNKP YNNKTDIWSL GCVLYELCTL KHPFEGNNLQ QLVLKICQAH FAPISPGFSR ELHSLISQLF QVSPRDRPSI NSILKRPFLE NLIPKYLTPE VIQEEFSHML ICRAGAPASR HAGKVVQKCK IQKVRFQGKC PPRSRISVPI KRNAILHRNE WRPPAGAQKA RSIKMIERPK IAAVCGHYDY YYAQLDMLRR RAHKPSYHPI PQENTGVEDY GQETRHGPSP SQWPAEYLQR KFEAQQYKLK VEKQLGLRPS SAEPNYNQRQ ELRSNGEEPR FQELPFRKNE MKEQEYWKQL EEIRQQYHND MKEIRKKMGR EPEENSKISH KTYLVKKSNL PVHQDASEGE APVQMEFRSC CPGWSAMARS WLTATSASQD IEKDLKQMRL QNTKESKNPE QKYKAKKGVK FEINLDKCIS DENILQEEEA MDIPNETLTF EDGMKFKEYE CVKEHGDYTD KAFEKLHCPE AGFSTQTVAA VGNRRQWDGG APQTLLQMMA VADITSTCPT GPDSESVLSV SRQEGKTKDP YSPVLILM
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分子量
81.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NEK5, short for NIMA-related kinase 5, is a serine/threonine kinase that plays a crucial role in cell cycle regulation and mitosis. Its importance has garnered attention in cancer research, particularly due to its overexpression in various malignancies. NEK5 is involved in centrosome function, spindle assembly, and chromosomal stability, making it essential for the proper division of cells. Dysregulation of NEK5 activity can lead to mitotic errors and contribute to tumorigenesis. The study of recombinant NEK5 protein has become pivotal for understanding its molecular functions and interactions within the cell. By producing NEK5 as a recombinant protein, researchers can investigate its enzymatic activity, identify potential substrates, and elucidate its role in oncogenic processes. Additionally, studying NEK5 in vitro provides insights into its mechanisms of action and helps in the development of targeted therapies aimed at inhibiting its function in cancer cells. Given the kinase's involvement in critical cellular processes, NEK5 represents a promising target for developing novel cancer treatments and enhancing our understanding of malignancy-related cellular dynamics.
