Analytical Data
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基因名
NOP5
- Application
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别名
Nucleolar protein 58. Nucleolar protein 5
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y2X3
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表达区间
1-529 aa
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氨基酸序列
MLVLFETSVGYAIFKVLNEKKLQEVDSLWKEFETPEKANKIVKLKHFEKFQDTAEALAAFTALMEGKINKQLKKVLKKIVKEAHEPLAVADAKLGGVIKEKLNLSCIHSPVVNELMRGIRSQMDGLIPGVEPREMAAMCLGLAHSLSRYRLKFSADKVDTMIVQAISLLDDLDKELNNYIMRCREWYGWHFPELGKIISDNLTYCKCLQKVGDRKNYASAKLSELLPEEVEAEVKAAAEISMGTEVSEEDICNILHLCTQVIEISEYRTQLYEYLQNRMMAIAPNVTVMVGELVGARLIAHAGSLLNLAKHAASTVQILGAEKALFRALKSRRDTPKYGLIYHASLVGQTSPKHKGKISRMLAAKTVLAIRYDAFGEDSSSAMGVENRAKLEARLRTLEDRGIRKISGTGKALAKTEKYEHKSEVKTYDPSGDSTLPTCSKKRKIEQVDKEDEITEKKAKKAKIKVKVEEEEEEKVAEEEETSVKKKKKRGKKKHIKEEPLSEEEPCTSTAIASPEKKKKKKKKRENED
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分子量
86 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NOP5, also known as NOP56, is a ribonucleoprotein that plays a crucial role in the biogenesis of ribosomal RNA and the assembly of ribosomes, essential processes for protein synthesis in all eukaryotic cells. Recent studies have highlighted its significance in various cellular functions, including cell proliferation, stress response, and the regulation of gene expression. Given its involvement in ribosome biogenesis, NOP5 is of particular interest in cancer research, as abnormalities in ribosomal components are often linked to tumorigenesis and cancer progression. Moreover, NOP5's interaction with other nucleolar proteins hints at its potential role in the coordination of cellular stress responses and ribosomal integrity. The reconstitution and characterization of NOP5 as a recombinant protein are essential for understanding its structural and functional properties. This research can pave the way for investigating NOP5's potential targets for therapeutic intervention, especially in cancer where its regulatory pathways may become altered. By studying NOP5 in a controlled laboratory setting, researchers aim to elucidate its mechanism of action, further delineate its role in ribosome assembly, and explore its implications in diseases associated with ribosomal dysfunction. Ultimately, this work contributes to the broader understanding of nucleolar function and its importance in cellular homeostasis and disease mechanisms.
