Analytical Data
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Gene name
NSUN3
- Application
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Alternative Names
6720484A09Rik; AU022521; FLJ22109; FLJ22609; MST077; MSTP077; NOL1/NOP2/Sun domain family 3; NOL1/NOP2/Sun domain family member 3; NOP2/Sun domain family member 3; NSUN3; NSUN3_HUMAN; OTTMUSP00000028633; Putative methyltransferase NSUN3; UG0651E06
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H649
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Expression Region
1-340 aa
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AA Sequence
MLTQLKAKSE GKLAKQICKV VLDHFEKQYS KELGDAWNTV REILTSPSCW QYAVLLNRFN YPFELEKDLH LKGYHTLSQG SLPNYPKSVK CYLSRTPGRI PSERHQIGNL KKYYLLNAAS LLPVLALELR DGEKVLDLCA APGGKSIALL QCACPGYLHC NEYDSLRLRW LRQTLESFIP QPLINVIKVS ELDGRKMGDA QPEMFDKVLV DAPCSNDRSW LFSSDSQKAS CRISQRRNLP LLQIELLRSA IKALRPGGIL VYSTCTLSKA ENQDVISEIL NSHGNIMPMD IKGIARTCSH DFTFAPTGQE CGLLVIPDKG KAWGPMYVAK LKKSWSTGKW
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Molecular Weight
38.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NSUN3 is a member of the NOP2/Sun RNA methyltransferase family, which has garnered significant interest in recent years due to its potential role in cellular processes such as growth, differentiation, and disease progression. This protein is primarily known for its involvement in mitochondrial RNA modification, specifically in the methylation of cytidine residues in mitochondrial coding and non-coding RNAs. Given the critical function of mitochondria in energy production and apoptosis, understanding NSUN3's role can provide insights into various mitochondrial disorders and metabolic diseases. Furthermore, aberrations in NSUN3 expression have been implicated in certain cancers, hinting at its potential as a biomarker or therapeutic target. The recombinant expression of NSUN3 enables detailed biochemical and structural studies, helping to elucidate its enzymatic mechanisms and interactions with other molecular partners. Research efforts thus focus on characterizing the functional properties of NSUN3 through recombinant protein studies, which can ultimately pave the way for novel approaches in treating diseases linked to mitochondrial dysfunction and aberrant RNA modifications.
