Analytical Data
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Gene name
NT5C3L
- Application
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Alternative Names
NT5C3B; NT5C3L7-methylguanosine phosphate-specific 5'-nucleotidase; 7-methylguanosine nucleotidase; EC 3.1.3.91; Cytosolic 5'-nucleotidase 3B; Cytosolic 5'-nucleotidase III-like protein; cN-III-like protein; EC 3.1.3.5; N(7)-methylguanylate 5'-phosphatase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q969T7
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Expression Region
1-300 aa
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AA Sequence
MAEEVSTLMK ATVLMRQPGR VQEIVGALRK GGGDRLQVIS DFDMTLSRFA YNGKRCPSSY NILDNSKIIS EECRKELTAL LHHYYPIEID PHRTVKEKLP HMVEWWTKAH NLLCQQKIQK FQIAQVVRES NAMLREGYKT FFNTLYHNNI PLFIFSAGIG DILEEIIRQM KVFHPNIHIV SNYMDFNEDG FLQGFKGQLI HTYNKNSSAC ENSGYFQQLE GKTNVILLGD SIGDLTMADG VPGVQNILKI GFLNDKVEER RERYMDSYDI VLEKDETLDV VNGLLQHILC QGVQLEMQGP
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Molecular Weight
34.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NT5C3L, or nucleoside triphosphate diphosphate kinase 3-like, is a critical enzyme involved in nucleotide metabolism and is known for its role in maintaining cellular nucleotide balance and signaling. Research has increasingly focused on NT5C3L due to its implications in various cellular processes, including cellular stress responses and DNA repair mechanisms. Mutations or dysregulation of NT5C3L have been associated with several pathologies, including cancer, where it may contribute to the altered metabolic landscape of tumor cells. The recombinant production of NT5C3L has become a pivotal area of study, as it allows for the detailed investigation of the enzyme's structure-function relationship, enzymatic activity, and regulatory mechanisms. By utilizing advanced techniques such as protein engineering and purification, researchers aim to produce high yields of functional NT5C3L, facilitating biochemical assays and potential therapeutic applications. Furthermore, characterizing the protein's interactions with nucleotides and other cellular factors can elucidate its role in health and disease, paving the way for novel therapeutic strategies targeting nucleotide metabolism. Overall, the study of NT5C3L as a recombinant protein not only enhances our understanding of nucleotide homeostasis but also contributes to the broader field of metabolic disease research.
