Analytical Data
-
Gene name
TIPIN
- Application
-
Alternative Names
TIPIN；TIMELESS-interacting Protein
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9BVW5
-
Expression Region
1-301aa
-
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLEPQEN GVIDLPDYEH VEDETFPPFP PPASPERQDG EGTEPDEESG NGAPVPVPPK RTVKRNIPKL DAQRLISERG LPALRHVFDK AKFKGKGHEA EDLKMLIRHM EHWAHRLFPK LQFEDFIDRV EYLGSKKEVQ TCLKRIRLDL PILHEDFVSN NDEVAENNEH DVTSTELDPF LTNLSESEMF ASELSRSLTE EQQQRIERNK QLALERRQAK LLSNSQTLGN DMLMNTPRAH TVEEVNTDED QKEESNGLNE DILDNPCNDA IANTLNEEET LLDQSFKNVQ QQLDATSRNI TEAR
-
Molecular Weight
37 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TIPIN (Tipin, a protein involved in DNA replication) is a crucial component of the cellular mechanisms that ensure the fidelity and efficiency of DNA replication. This protein is known to interact with the replication fork and plays an essential role in coordinating DNA synthesis with other cellular processes, including DNA damage response and repair. Research has shown that TIPIN interacts with several key proteins involved in replication, such as ATR and other components of the replication machinery, highlighting its integral role in maintaining genomic stability. Disruptions in TIPIN function can lead to DNA replication stress and contribute to genomic instability, which is frequently associated with various diseases, including cancer. Consequently, understanding TIPIN’s structural and functional characteristics has significant implications for designing therapeutic strategies aimed at targeting replication-related pathways in cancer cells. Recent studies have focused on the recombinant expression and characterization of TIPIN to elucidate its molecular interactions and regulatory mechanisms. By utilizing techniques like X-ray crystallography and nuclear magnetic resonance (NMR), researchers aim to gain insights into TIPIN's role in cellular responses to replication stress and its potential as a biomarker or therapeutic target in cancer treatment. The combination of biochemical assays and structural biology approaches will pave the way for a deeper understanding of TIPIN's contributions to DNA replication fidelity and its implications in oncogenesis, further establishing TIPIN as a critical player in the maintenance of genomic integrity.
