Analytical Data
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Gene name
APG5L
- Application
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Alternative Names
APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; APG5L
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H1Y0
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Expression Region
1-275aa
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AA Sequence
MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI TVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ KLFRPVAADG QLHTLGDLLK EVCPSAIDPE DGEKKNQVMI HGIEPMLETP LQWLSEHLSY PDNFLHISII PQPTD
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Molecular Weight
32.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
APG5L, or Autophagy Related 5-Like protein, has gained significant attention in recent years due to its pivotal role in autophagy, a cellular process essential for maintaining cellular homeostasis and responding to stress. Originally identified as a key component in the autophagic machinery, APG5L interacts with other autophagy-related proteins and is crucial for the formation of autophagosomes, which are cellular structures that engulf damaged organelles and proteins for degradation. Research indicates that dysregulation of APG5L and the autophagic process is implicated in various diseases, including neurodegenerative disorders, cancer, and infections. Therefore, studying the recombinant protein APG5L provides researchers with insights into its functional mechanisms and its potential therapeutic applications. By producing and characterizing recombinant APG5L in the lab, scientists aim to elucidate its precise roles in autophagy and its broader implications in health and disease. Understanding the biochemical pathways and interactions involving APG5L could not only enhance our knowledge of autophagy but also pave the way for novel interventions targeting diseases linked to autophagic dysfunction.
