Analytical Data
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Gene name
ATP6V1B1
- Application
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Alternative Names
Vacuolar proton pump subunit B 1; VATB; VATB1_HUMAN; VMA2; Vpp 3; Vpp3
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P15313
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Expression Region
1-513aa
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AA Sequence
MAMEIDSRPG GLPGSSCNLG AAREHMQAVT RNYITHPRVT YRTVCSVNGP LVVLDRVKFA QYAEIVHFTL PDGTQRSGQV LEVAGTKAIV QVFEGTSGID ARKTTCEFTG DILRTPVSED MLGRVFNGSG KPIDKGPVVM AEDFLDINGQ PINPHSRIYP EEMIQTGISP IDVMNSIARG QKIPIFSAAG LPHNEIAAQI CRQAGLVKKS KAVLDYHDDN FAIVFAAMGV NMETARFFKS DFEQNGTMGN VCLFLNLAND PTIERIITPR LALTTAEFLA YQCEKHVLVI LTDMSSYAEA LREVSAAREE VPGRRGFPGY MYTDLATIYE RAGRVEGRGG SITQIPILTM PNDDITHPIP DLTGFITEGQ IYVDRQLHNR QIYPPINVLP SLSRLMKSAI GEGMTRKDHG DVSNQLYACY AIGKDVQAMK AVVGEEALTS EDLLYLEFLQ KFEKNFINQG PYENRSVFES LDLGWKLLRI FPKEMLKRIP QAVIDEFYSR EGALQDLAPD TAL
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Molecular Weight
81.95 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATP6V1B1 encodes a subunit of the vacuolar ATPase (V-ATPase), an essential enzyme responsible for acidifying intracellular compartments and regulating various cellular processes. Mutations in the ATP6V1B1 gene are implicated in several human diseases, notably autosomal recessive renal tubular acidosis (AR-RTA) and related conditions affecting kidney function. Research on recombinant ATP6V1B1 protein is vital for understanding the structure-function relationship of V-ATPase, elucidating the mechanisms underlying such mutations, and exploring their impact on cellular pH homeostasis. The development of recombinant ATP6V1B1 enables researchers to produce the protein in significant quantities for biochemical characterization, kinetic studies, and crystallization, which can reveal insights into the enzyme's activity and regulation. Furthermore, studying the interactions between ATP6V1B1 and other V-ATPase components can enhance our understanding of the assembly and functional integrity of this multi-subunit complex. Overall, recombinant ATP6V1B1 is a crucial tool for advancing our knowledge of cellular acidification processes and their roles in health and disease, paving the way for potential therapeutic strategies targeting V-ATPase-related disorders.
